Kinetics of HK cleavage.

<p>HK (200 µg/ml) was incubated with (<b>A</b>) kallikrein (0.5 µg/ml), (<b>B</b>) rMASP-1 (50 µg/ml), or (<b>C</b>) rMASP-2 (30 µg/ml) in HBS buffer (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020036#s2" target="_blank">Methods</a>) at 37°C. Samples were taken periodically (time in minutes indicated), and 15 µl aliquots were run on SDS-PA gels under reducing conditions. The kinetic constants, determined by densitometry of the HK band followed by non-linear regression analysis, are found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020036#pone-0020036-t001" target="_blank">Table 1</a>.