RT-PCR analysis of <i>β</i>4GalT mRNAs in <i>Smad4^{co/co;Lck-cre}</i> mice.

<p>mRNA was isolated from purified B-cells (>95% CD19+), and RT-PCR was performed using primers specific for <i>β</i>4GalT1 to 7, and GAPDH. <i>β</i>4GalT 2 and 4 mRNA expression in <i>Smad4^{co/co;Lck-cre}</i> mice (n = 4, black column) were significantly reduced relative to controls (n = 4, white column). All samples were normalized to GAPDH levels. Data are expressed as mean±SEM; P values were calculated with <i>t</i> test. *P<0.05