Phylogenetic placement of the yeast isolates associated with <i>Doubledaya bucculenta</i>.

<p>(A) A neighbor-joining phylogeny inferred from DNA sequences of D1/D2 domain of 26S rRNA gene (530 bps). (B) A neighbor-joining phylogeny inferred from DNA sequences of EF-1α gene (709 bps). (C) A neighbor-joining phylogeny inferred from DNA sequences of ITS/5.8S rRNA gene (360 bps). Bootstrap values (1000 replicates) of 50% or higher are shown at the nodes. Sequence accession numbers are shown in brackets. Yeast isolates obtained from <i>D. bucculenta</i> and <i>Pleioblastus simonii</i> in this study are discriminated by sites of eggs and <i>P. simonii</i> internodes where the isolate was obtained, the number to specify an egg and the internode harboring the egg, and the localities where the isolates were sampled. Abbreviations: AE, the anterior end of an egg; LS, the lateral side of an egg; PE, the posterior end of an egg; OH, the edge of the inner opening of an oviposition hole. ^aMiyazaki Prefecture, Japan; ^bIbaraki Prefecture, Japan; ^ca yeast isolate with colony texture different from <i>Wickerhamomyces anomalus</i>, which was obtained at the inner opening of an oviposition hole.