<i>In</i><i>situ</i> hybridization for <i>Pax6</i> and <i>Tau-GFP</i> on coronal sections of E12.5 DTy54 embryos.

<p>Adjacent 10μm coronal sections were hybridized with a probe for <i>Pax6</i> (A,C,E,G) or <i>tau-GFP</i> (B,D,F,H). (A-H) <i>Pax6</i> and <i>tau-GFP</i> expression throughout caudal to rostral levels of the forebrain. (G-I) Direct comparison of <i>Pax6</i> and <i>tau-GFP</i> transcripts in the ventral telencephalon. Image in G is colour-inverted and superimposed on that in H to produce the merged image in I. Arrow indicates the PSPB. Strong <i>tau-GFP</i> expression was expanded into the subpallial side of the PSPB, while <i>Pax6</i> expression levels dropped sharply at the PSPB. In addition, only Pax6 mRNA but not <i>tau-GFP</i> mRNA was expressed in the postmitotic cells in the basolateral telencephalon (asterisk in G). LCS, lateral cortical stream. n=8 DTy54 embryos. Scale bars: 200µm.