Double immunostaining for Pax6 and tau-GFP on coronal sections at caudal to rostral levels through the forebrain from E12.5 Y001 embryos.

<p>(A, D, G) Pax6 expression is reflected by red fluorescence. (B, E, H) In the diencephalon, tau-GFP is only expressed in the prethalamus (PTH). The tau-GFP expression in the cortex is dramatically reduced in comparison with the endogenous Pax6 expression. tau-GFP highly labeled cells are present in the ventral telencephalon, (C, F, I) Direct comparison of tau-GFP and Pax6 expression revealed that tau-GFP expression only exhibits a partial Pax6 pattern, with some notable discrepancies. (J) tau-GFP expression in the pretectum (PT) is lost. (K) A band of Pax6 only expressing cell is located adjacent to the tau-GFP labeled cellular processes in the prethalamus. (L) The expression of tau-GFP in the cortex (CTX) is very weak. (M) tau-GFP heavily labeled cells are present in the mantle region of the ventral telencephalon. (N) A subset of tau-GFP labeled migratory cells co-expresses Pax6. (O) Pax6 single expressing cells are present within the amygdaloid (AMY) region. Abbreviations as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080208#pone-0080208-g002" target="_blank">Figure 2</a>. n=8 Y001 embryos Scale bars: A-I, 200µm; J-O, 100 µm.