Analysis of noise and response properties of topologies 14 and 30 assuming monofunctional HK. A & B.

<p>Signal-response curve for topologies 14 (A) and 30 (B). The x-axis corresponds to the signal input to the system, which in the model is approximated by varying the HK auto-phosphorylation rate constant, <i>k_s</i>. The y-axis corresponds to the simulated mean of the fraction of phosphorylated RR, calculated using PRISM model checker (see <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003322#s4" target="_blank"><i>Methods</i></a>). The solid and dashed curves show the results obtained from constraining the system parameters in the hyperbolic and sigmoidal regimes respectively. <b>C & D.</b> Noise levels in phosphorylated RR for topologies 14 (C) and 30 (D). The x-axis shows the signal input to the system, taken to be the HK auto-phosphorylation rate constant, <i>k_s</i>. The y-axis shows the standard deviation over mean of the fraction of phosphorylated RR at steady state, both calculated using PRISM model checker (see <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003322#s4" target="_blank"><i>Methods</i></a>). <b>E & F.</b> Box plots showing the distribution of response times for topologies 14 (E) and 30 (F) as measured from hyperbolic and sigmoidal regimes. For each topology, the response time is measured for 100 randomly selected parameter sets from the hyperbolic and sigmoidal regimes.