Dose-response analysis for the dual step phosphorylation model.

<p>Transfer functions for each of the three layers of the MAPK cascade (A-C), obtained considering for each layer i) the isolated module (Is, dotted blue), ii) a mechanistic implementation of the model (Seq, dashed-turquoise) and iii) the mathematical composition of isolated response functions (Non-Seq, continous red). The corresponding response coefficient curves are shown in panels (D-F). Turquoise dashed vertical lines show the <i>X</i>10_<i>i</i> and <i>X</i>90_<i>i</i> values of each layer (i.e. mechanistic scheme), while red solid vertical lines mark the layer’s <i>X</i>10_<i>i</i> and <i>X</i>90_<i>i</i> associated to the composition of response curves of each module (i.e. <i>F</i>^{<i>non</i>—<i>seq</i>}).