The Smc6 protein localizes to meiotic chromosomes during prophase in a cohesin- dependent manner.

<p>Cells were isolated at the indicated time points and surface-spread to detect epitope-tagged proteins Smc6 (Smc6-13Myc, green) and Rec8 (Rec8-3HA, red) using standard immunofluorescence techniques. DNA visualized with DAPI shown in grey. (<b>A</b>) Wild type (CB1361). (<b>B</b>) <i>rec8Δ</i> (CB1411). (<b>C</b>) Enlarged image of a representative cell from the wild type (CB1361) at the 4 h time point. Solid arrows indicate an Smc6 site at a weaker-staining Rec8 site. Dashed arrows indicate a strong Smc6 site on top of a strong Rec8 site.