<p><b>A.</b> SYP-1 immunostaining of representative diakinesis nuclei of wild type, <i>smc-5(ok2421)</i>, <i>him-6(ok412)</i>, and <i>smc-5(ok2421); him-6(ok412)</i> mutants. White arrowheads indicate the remaining SYP-1 staining. <b>B.</b> Representative images of nuclei of diakinesis oocytes stained with an antibody recognizing the chromosome axis component HIM-3. Red arrows indicate the not well-compacted bivalents. Scale bars: 2 μm.</p
<p>(<b>A</b>) Set-up of the soft-shift method. Cells were shifted to non-permissive temperature (33°...
<p>A) DAPI staining of haploid diakinesis. B) Immunolocalization of MLH1 in wild-type haploid. C) Me...
<p>(A–H) Shown are surface spread meiotic chromosomes from the indicated genotypes of males. Antibod...
<p>(A) Co-immuno labeling for Smc6-myc13 and cohesin subunit Rec8-HA3 of a spread, leptotene nucleus...
<p><b>A.</b> Representative images taken from time-lapse recordings of GFP-Histone H2B expressing em...
<p>(A) Schematic representation of the Smc5/6-Mms21 subunits. (B) Schematic of the temperature shift...
<p>Cells were isolated at the indicated time points and surface-spread to detect epitope-tagged prot...
<p>(A) Western blot analysis of Mec1 substrates, Hop1 (pT318) and H2A (pS129, γH2A). Clb1 and Clb3 a...
<p>(A) SYP-1 staining of representative diplotene nuclei. Scale bars are 2 µm. (B) HTP-1/2 (red) and...
(A) Representative images of wild-type and zhp-4(vv96) diakinesis nuclei stained with α-AIR-2 (green...
<p>Analysis of whole-mounted germlines from wild type and <i>ztf-8</i> mutants co-stained with DAPI ...
SMC complexes include three major classes: cohesin, condensin and SMC5/6. However, the localization ...
<p>Developing ovules were prepared and immunostained with an antibody against DMC1, which is specifi...
Meiosis, a specialized cell division with a single cycle of DNA replication round and two consecutiv...
<p>(A) In wild type cells, Smc5/6 is present and ensures the formation of IH-dHJs either directly or...
<p>(<b>A</b>) Set-up of the soft-shift method. Cells were shifted to non-permissive temperature (33°...
<p>A) DAPI staining of haploid diakinesis. B) Immunolocalization of MLH1 in wild-type haploid. C) Me...
<p>(A–H) Shown are surface spread meiotic chromosomes from the indicated genotypes of males. Antibod...
<p>(A) Co-immuno labeling for Smc6-myc13 and cohesin subunit Rec8-HA3 of a spread, leptotene nucleus...
<p><b>A.</b> Representative images taken from time-lapse recordings of GFP-Histone H2B expressing em...
<p>(A) Schematic representation of the Smc5/6-Mms21 subunits. (B) Schematic of the temperature shift...
<p>Cells were isolated at the indicated time points and surface-spread to detect epitope-tagged prot...
<p>(A) Western blot analysis of Mec1 substrates, Hop1 (pT318) and H2A (pS129, γH2A). Clb1 and Clb3 a...
<p>(A) SYP-1 staining of representative diplotene nuclei. Scale bars are 2 µm. (B) HTP-1/2 (red) and...
(A) Representative images of wild-type and zhp-4(vv96) diakinesis nuclei stained with α-AIR-2 (green...
<p>Analysis of whole-mounted germlines from wild type and <i>ztf-8</i> mutants co-stained with DAPI ...
SMC complexes include three major classes: cohesin, condensin and SMC5/6. However, the localization ...
<p>Developing ovules were prepared and immunostained with an antibody against DMC1, which is specifi...
Meiosis, a specialized cell division with a single cycle of DNA replication round and two consecutiv...
<p>(A) In wild type cells, Smc5/6 is present and ensures the formation of IH-dHJs either directly or...
<p>(<b>A</b>) Set-up of the soft-shift method. Cells were shifted to non-permissive temperature (33°...
<p>A) DAPI staining of haploid diakinesis. B) Immunolocalization of MLH1 in wild-type haploid. C) Me...
<p>(A–H) Shown are surface spread meiotic chromosomes from the indicated genotypes of males. Antibod...