VAChT KD^HOM NMJs have normal morphology but altered SVs distribution in the absence of stimulus.

<p>A and B– Representative images of nerve terminal profile from VAChT WT and VAChT KD^HOM mice in the absence of stimulation showed a altered SVs distribution from the active zone within the circles: 50 and 300 nm from the membrane, small and big circles respectively. Scale bar = 500 nm. Magnification 50.000x. C– Graph showing the area of the presynaptic terminals in μm². D– Graph comparing the total postsynaptic membrane lenght (μm). E – Graph of the ratio SVs/area of presynaptic terminal in μm². F– Graph showing the average number of SVs located at different distances from the presynaptic active zones. G and H– Four serial sections of the profile of NMJs of VAChT WT (G1–G4) and VAChT KD^HOM (H1–H4) mice showing the altered SVs distribution in the active zone (* represent depletion areas of SVs) of motor terminals of VAChT KD^HOM in the absence of stimulus. Scale bar = 500 nm. Magnification 50.000x. (n = 5 individual animals per genotype. * p<0.05).