Isolation of a novel CSC/CIC molecule, SMCP.

<p><b>A. Isolation of SP cells from LHK2, MCF7 and SW480 cells.</b> Lung (LHK2), colon (SW480) and breast (MCF7) cancer cells were stained with Hoechst₃₃₃₄₂ dye in the absence (upper panel) or presence (lower panel) of verapamil. All cell lines were analyzed by a cell sorter. <b>B. Expression profiles of stem cell markers in SP cells and MP cells.</b> Expression of stem cell markers (<i>SOX2</i>, <i>POU5F1</i> and <i>NANOG</i>) in SP cells and MP cells derived from LHK2, SW480 and MCF7 cells was determined. <i>GAPDH</i> was used as an internal positive control. <b>C. Quantitative real-time PCR analysis of SOX2 mRNA expression in SP and MP cells.</b> The MP cells were used for the control, which was set as 1.0. Data are expressed as mean + SD of relative values compared to MP cells. <b>D. Expression of </b><b><i>SMCP</i></b><b> in SP and MP cells.</b> Expression of <i>SMCP</i> in SP cells and MP cells derived from LHK2, SW480 and MCF7 cells was determined. <b>E. SMCP is localized in mitochondria.</b> LHK2 cells transfered with GFP-fused SMCP gene were fixed and stained with Mitotracker Red followed by DAPI and then visualized by laser confocal microscopy (Magnification, ×200). Green indicates GFP-fused SMCP. Red indicates mitochondria. Blue indicates nucleus.