Influence of adhesion inhibitors on <i>in</i><i>vitro</i> matrix formation.

<p>Incorporation of rhodamine-labelled fibronectin into the extracellular matrix of fibroblasts was visualized by fluorescence microscopy, after 12 or 24 hours of incubation in medium alone (NI, A, F) or medium containing immobilized GST (CB, B, G), MAM7 (C, H), F1 (D, I) or FnBPA (E, J) derived peptides. Background fluorescence of unlabelled cells at t=0 hours (K). Scale bar, 10 μm. Images are representative of a set of three experiments, with at least three frames taken per replicate. Incorporation of rhodamine-fibronectin into the extracellular matrix in medium alone (NI) or medium containing immobilized GST (CB), MAM7, F1 or FnBPA derived peptides was quantified after 12 hours (grey) and 24 hours (black) incubation using fluorescence plate assays (L). Values significantly different from the control (p<0.01 according to student t-test) are indicated (*).