Troglitazone treatment dampens K562 cell adhesion RGD containing extracellular matrix proteins fibronectin, FN-120, and vitronectin.

<p>(A) Microtiter wells were coated with 5 μg/mL fibronectin, FN-120, or vitronectin. K562 cells cultured for 72 hrs in the presence of vehicle (<i>white bars</i>) or 15 μM troglitazone (<i>black bars</i>) were added to wells (9×10⁴ cells/well) in Hepes-Tyrodes buffer containing 1 mM MnCl₂. (B) K562 cells cultured for 72 hrs with vehicle (<i>white bars</i>) or various concentrations of troglitazone (<i>black bars</i>) were added to wells coated with 5 μg/mL FN-120, and adhesion assays were repeated. Specific numbers of adherent cells/well were obtained by correlating experimental absorbance values to a standard curve. Adhesion values obtained with wells coated exclusively with BSA were considered as background values for each experimental condition and were subtracted before reporting final values. Adherent cells/well = adherent cells_(protein) – adherent cells_(BSA). Results are expressed as means ± SD, n = 6, Student’s t-test (p<0.01) (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093005#pone-0093005-g001" target="_blank">Figure 1A</a>) or LSD (P = 0.01) (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093005#pone-0093005-g001" target="_blank">Figure 1B</a>) was used to test for differences among groups. Means followed by the same letter are not significantly different.