Phylogenetic analysis of bacilli TatCd and TatCy proteins and <i>tatC</i> operon conservation.

<p>Bayesian analysis was performed to generate the dendrogram provided on the left hand side of the panel and shows the homology between 57 representative bacilli TatC sequences including characterized <i>B. subtilis</i> TatCd and TatCy seed sequences (underlined). Nodes of importance are indicated by labelled black circles based on its relationship to either TatCd (d1–d3) or TatCy (y1, y2, y3a, y3b- y7). <i>Bacillus</i> species marked by an asterisk ‘Tn’ indicate that its corresponding <i>tatC</i> operon was adjacent to transposons or integron sequences. The arrow and box line diagrams shown on the right hand side of the dendrogram summarize <i>tatC</i> operon conservation for the indicated species. White filled arrows/boxes represent conserved genes located upstream and downstream from <i>tatA</i> (grey arrows) and <i>tatC</i> (black filled arrows). The direction of each arrow represents the open read frame direction relative to <i>tatC</i> and boxes represent genes present in either reading frame. Boxes and arrows with dotted lines indicate genes with moderate conservation 50–80% of <i>tatC</i> loci and solid lines indicate ≥80% gene conservation. The triangle symbol located on the dendrogram points out the division between the TatCd and TatCy clades. The symbol (≠) after <i>L. spaericus</i> indicates that it shares a similar <i>tatC</i> operon arrangement to that shown for sequences in the distal node of y1. Full names for abbreviated genes are listed as follows: alkaline phosphatase (<i>phoD</i>), pyrrolidone-carboxylate peptidase (<i>pcp</i>), domain of unknown function protein (DUF839), lipid esterase A (<i>lipA</i>), hypothetical lipid binding phosphotransferase (<i>degV</i>), domain of unknown function protein (DUF2535), arabinose family transcriptional regulator (<i>araC</i>), dihydrofolate reductase A (<i>dfrA</i>), thymidylate synthase A (<i>thyA</i>), periplasmic binding protein domain 3 (<i>pbp</i>), azoreductase (<i>yvaB</i>), 1-acyl-sn-glycerol 3-phosphate acyltransferase (<i>plsC</i>), 5-formyltetrahydrofolate cyclo ligase (5′FTHF), riboendonuclease liver perchloric acid-soluble protein (L-PSP) (<i>yjgF</i>), molybdenum cofactor biosynthesis protein C (<i>moaC</i>), molybdopterin biosynthesis protein (<i>mogA</i>/<i>moeA</i>), small and large subunit chaperones (<i>groES</i>/groEL), mercury resistant transcriptional regulator (<i>merR</i>), gluconate 2 dehydrogenase subunit 3 (GADH), gluconate 2 dehydrogenase flavoprotein (<i>betA</i>), ABC transporter ATP-binding protein- Elongation factor 3 (<i>uup</i>), redox sensing transcriptional repressor (<i>rex</i>), abortive infection CAAX protease protein (<i>abi</i>), iron dependent DyP-type peroxidase (<i>efeB/ywbN</i>), DUF4305 a 53 amino acid lipoprotein (<i>ydkI</i>), glycoprotein endopeptidase peptidase M22 (<i>yeaZ</i>), transcriptional regulator of sugar metabolism (<i>glpR</i>), high-affinity Fe²⁺/Pb²⁺ permease (FTR1/<i>efeU</i>).