HTS screen of the Spectrum library.

<p>Raw fluorescence data from the Spectrum screen were normalized to the assay controls on each plate. The normalized data are shown as circles with each assay plate being a different color. The solid lines represent the average value of the positive (▴) and negative (▾) controls. The dashed black lines represent one standard deviation from the positive and negative controls. The red dashed line represents 5 standard deviations below the average negative control. Compounds below this cutoff were considered primary hits. 57 of the 2320 compounds were identified as hits.