Reproducible and stable signals from HTS of the chemical library.

<p>(A) Schematic of assay plate map used in screening the chemical compound library. (B) Reproducibility of raw data between duplicate assay plates for control wells containing IFN-β (0–200 U/ml) and test wells containing compound plus IFN-β (5 U/ml). Dashed lines indicate median ± SD of each data set from the test wells. (C) Signal consistency through a full screen of 56 assay plates. Values represent replicate A of assay plate 1 (the first plate) and plate 55 (the last plate). Box plots represent median and 25^th and 75^th percentile, and whiskers indicate minimum and maximum of data. Arrows indicate values from test wells.