Adenovirus pVII propeptide sequence induces proteasomal degradation.

<p>(<b>A</b>) Schematic representation of the Ad5 precursor pVII, mature VII, propeptide and 1-24Gal4 proteins. (<b>B</b>) Repression of Gal4 DNA binding site containing luciferase reporter by the 1-24Gal4 protein in HEK293T cells. Data shown as relative activation of Firefly luciferase reporter gene after normalization to Renilla luciferase control gene. (<b>C</b>) MG132 treatment increases the 1-24Gal4 protein levels. HEK293T cells expressing the Gal4 and 1-24Gal4 proteins were treated with 25 μM of MG132 for 5 hours. Protein expression was detected by Western blotting (WB) using anti-Gal4 and anti-Actin antibodies. Presence of the Gal4 protein signal in 1-24Gal4 transfected cells is due to internal translation initiation of the Gal4 protein encoded by the 1-24Gal4 plasmid.