DPQ treatment attenuates LPS-induced acute lung injury.

<p>To induce the ALI, 8–10 weeks old male C57BL/6 mice were intraperitoneally injected with LPS at 7.5 mg/kg. After 30 minutes, 1 or 10 μg/kg of DPQ was administrated. (A) Representative micrographs of H & E staining (DPQ 10 μg/kg). Lung tissues were fixed by formalin and embedded into paraffin. Following sectioning with 5 μm thickness, the sections were stained with H & E. Green arrows indicate the infiltrated neutrophils. Scale bar: 50 μm. (B) Quantitative analysis of recruited neutrophils in lungs at 6 hours after LPS challenge. The average number of infiltrated neutrophils are shown. (C) MPO activity assay of lung tissue. Lung tissues were homogenized, and the MPO activity of homogenates were measured with microplate reader at 460 nm. Data are expressed as mean ± SEM; n = 5; *<i>P</i><0.05 versus Basal; #<i>P</i><0.05 versus LPS alone.