Activation of the canonical Wnt pathway increases MPP and MEP generation.

<p>(A) Cells from CONDITION 2 of the differentiation, (DP-βC +/- Tet and E14 ES cells +/- BIO) were cultured in a MPP cocktail for 7 days prior to multi-parameter flow cytometry analysis of total cell population from CONDITION 3. Live cells from CONDITION 3 of the differentiation were gated and the expression of the hematopoietic progenitor markers, Sca1^hic-Kit^lo and Sca1^loc-Kit^hi were profiled along with the megakaryocytic marker CD41 and transferrin receptor CD71. (B) MPP population (CONDITION 3) were then replated for an additional 7 days and analyzed for the same panel of markers outlined in (A). Images are representative of 3 independent experiments.