The effect of noncanonical Wnt pathway on the expression of specific markers of alveolar epithelial cells in mMSCs differentiating into AT II cells.

<p>The expression of pro-SPC protein (<b>A</b>) and SPB, SPC, SPD and AQP5 mRNA (<b>B</b>) in mMSCs after 10 days of differentiation driven by co-culture with MLE-12 cells and SAGM with supplementation of 500 ng/ml Wnt5a plus 5 µmol/L SP600125 or 2.5 µmol/L GF109203X were evaluated with western blotting and qRT-PCR. (<i>n</i> = 3; *<i>P</i><0.05 <i>vs</i> Control; #<i>P</i><0.05, ##<i>P</i><0.01 <i>vs</i> DMSO control; &<i>P</i><0.05, &&<i>P</i><0.01 <i>vs</i> differentiation + DMSO + Wnt5a + GF109203X- SP600125-).