Katanin p60 was bundled with microtubules by contractility of the contractile ring and distributed in a microtubule-dependent manner.

<div><p>A. 3Y1 cells were treated with 10 µM blebbistatin for 1 h and then labeled for katanin p60 (green), actin (red), and DNA (blue). Merge indicates merged images of katanin p60, actin, and DNA, showing the localization of katanin p60, microtubules, and the contractile ring at anaphase (Ana) and telophase (Telo) during mitosis. Scale bars: 10 µm. Samples were fixed in methanol and analyzed by confocal laser scanning fluorescence microscopy (FV-1000D; Olympus).</p> <p>B. 3Y1 cells were treated with 10 µM blebbistatin for 1 h and then labeled for katanin p60 (green), β-tubulin (red), and DNA (blue). Merge indicates merged images of katanin p60, β-tubulin and DNA, showing the localization of katanin p60 and microtubules at telophase (Telo) during mitosis. Cross-section showing vertical images of the distributions of katanin p60 (green), β-tubulin (red), and DNA (blue). Green arrowheads indicate vertical analysis point corresponding to the “Center” of the plane where katanin p60 was present. Red arrowheads indicate vertical analyses points corresponding to “Right” and “Left” at both sides neighboring the plane where katanin p60 was present. “B” and “T” indicate “Bottom” and “Top” of the cell, respectively. Scale bars: 10 µm. Samples were fixed in methanol and analyzed by confocal laser scanning fluorescence microscopy (FV-1000D; Olympus). </p> <p>C. 3Y1 cells were treated with 10 µM nocodazole for 30 min, and then labeled for katanin p60 (green), β-tubulin (red), and DNA (blue). Merge indicates merged images of katanin p60, β-tubulin, and DNA at prophase – metaphase (Pro – Meta) and at cytokinesis (Cytokinesis). Both katanin p60 and microtubules disappeared. Scale bars: 10 µm. Samples were fixed in methanol and analyzed by fluorescence microscopy (Axioskop II; Carl Zeiss).</p>