Subcellular localization of endogenous katanin p60.

<div><p>A. 3Y1 cells labeled for katanin p60 (green), β-tubulin (red), and DNA (blue). Merge indicates merged images of katanin p60, β-tubulin, and DNA, showing the localization of katanin p60 during mitosis. Pro, Meta, Ana, Telo, and Cytokinesis indicate images of prophase, metaphase, anaphase, telophase, and cytokinesis, respectively. Scale bars: 10 µm. Samples were fixed in methanol and analyzed by confocal laser scanning fluorescence microscopy (FV-1000D; Olympus).</p> <p>B. Higher magnification micrograph around the central spindle in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080392#pone-0080392-g002" target="_blank">Figure 2</a>. Telophase Merged image is shown in the upper left (Central spindle). Vertical analysis images of the distribution of katanin p60 (green), β-tubulin (red), and DNA (blue). Merge indicates merged images of katanin p60, β-tubulin, and DNA, showing a cross-section of the thin yellow line in the Midbody image. Density indicates the images of the density of katanin p60 and of ß-tubulin (microtubules) in cross-section images. White arrowheads indicate the same position of images. Scale bars: 2 µm. Analyses were performed by confocal laser scanning fluorescence microscopy (FV-1000D; Olympus).</p> <p>C. Micrographs around the contractile ring. 3Y1 cells labeled for katanin p60 (green), actin (red), and DNA (blue). Merge indicates merged images of katanin p60, actin, and DNA, showing the localization of katanin p60 and the contractile ring at telophase. Cross-section analysis of the region between white arrowheads is shown in the Cross-section row. Scale bars: 5 µm (Horizontal) and 2.5 µm (Cross-section). Analyses were performed by confocal laser scanning fluorescence microscopy (FV-1000D; Olympus).</p>