Effect of chronic hypoxia on expression of GTPCH-1, DHFR and eNOS in lungs and intrapulmonary arteries.

<p>Graphs showing quantified data normalized to β-actin and representative Western-blotting for (<i>A</i> and <i>B</i>) GTPCH-1, (<i>C</i> and <i>D</i>) DHFR, and (<i>E</i> and <i>F</i>) total eNOS, in lungs (ACE) and intrapulmonary arteries (BDF), from mice exposed to normoxia (NX) or 10, 21 and 40 days of hypoxia (HX 10d, HX 21d and HX 40d, respectively). (<i>G</i>) Expression of eNOS at the mRNA level by quantitative RT-PCR in lung from mice exposed to normoxia (NX) or 21 days of hypoxia (HX 21d). Results are expressed as mean ± SEM from 5 (Western-blotting) to 9 (RT-PCR) experiments. * p<0.05, ** p<0.01, *** p<0.001, compared to NX.