Bioinformatic evaluation of the impact of W151C mutation and multiple-sequence alignment analysis.

<p>(A) The PolyPhen-2 program was used to predict the possible impact of the amino acid substitution on the structure and function of βB2-crystallin. The score from PolyPhen-2 analysis is 1.00, which means that the W151C mutation is predicted to probably damage to the structure and function of βB2-crystallin. (B) The outcome from SIFT shows that the amino acid change is probably damaging to the function of protein, with the score is 0.00 and the median information content is 2.73. (C) Multiple-sequence alignment in CRYBB2 from different species reveals that codon 151, where the mutation (p. W151C) occurred, is highly conserved (highlighted in green, the mutant in red).