SDS-PAGE analysis of the digestion products of the Cry8E toxin and Cry8E toxin-DNA complex by proteinase K at 37°C for 5 min.

<p>A. Digestion of the Cry8E toxin by proteinase K. B. Digestion of the Cry8E toxin-DNA complex by proteinase K. Lane 1, molecular mass marker; Lanes 2–7, products of the digestion of the toxin or toxin-DNA complex by proteinase K at protein (weight/weight) ratios of 1∶5, 1∶10, 1∶20, 1∶30, 1∶40, and 1∶50, respectively; Lane 8A, the Cry8E toxin; and Lane 8B, the Cry8E toxin-DNA complex. A t-test was used to analyze statistical differences of the mean values of the digestion products of the Cry8E toxin-DNA complex by Proteinase K when compared with the Cry8E toxin, as indicated in the right table.