Electrophoretic analysis of the purified Cry8Ca2 toxin and toxin-DNA complex.

<p>A. Cry8Ca2 toxin purified using Resource-Q. A1. Elution profile of the Cry8Ca2 toxin. Peak 1 is the fraction containing the Cry8Ca2 toxin. A2. Electrophoretic analysis of the Cry8Ca2 toxin by SDS-PAGE. Lane 1, purified Cry8Ca2 toxin. A3. DNA analysis by 0.7% agarose gel electrophoresis. Lane 1, DNA associated with Cry8Ca2 protoxin. Lane 2, DNA associated with the Cry8Ca2 toxin after chymotrypsin digestion. Lane 3, peak 1 fraction of the Cry8Ca1 toxin. Lane 4, peak 2 fraction of the Cry8Ca1 toxin. B. Cry8Ca2 toxin purified using Superdex 200. B1. Elution profile of the Cry8Ca2 toxin-DNA complex. The peak indicated by the arrow is the fraction containing the Cry8Ca1 toxin-DNA complex. B2. Electrophoretic analysis of the Cry8Ca2 toxin and Cry8Ca2 toxin-DNA by SDS-PAGE. Lane 1, purified Cry8Ca2 toxin (peak fraction of B1 digested with DNase I and collected using Superdex 200) and Lane 2, purified Cry8Ca2 toxin-DNA complex (peak fraction of B1). B3. Analysis of the DNA associated with the Cry8Ca2 toxin and the Cry8Ca2 toxin-DNA complex using 0.7% agarose gel electrophoresis. Lane M, DNA Marker (λ DNA/<i>Eco</i>130I); Lane 1, DNA preparation from the Cry8Ca2 toxin; and Lane 2, DNA associated with the Cry8Ca2 toxin-DNA complex.