Differentiation of new-born NPCs to neuronal cell types induced by soya-Ι administration.

<p>A. Confocal laser scanning microscopic images of neuronal cells merged with isotype cell markers in the granular cell layer of brain slices (green: BrdU-positive cells, red: VGluT1, GAD67, and NeuN). Arrow heads = non-merged cells, arrows = merged cells (the image below left; BrdU and GAD67-double positive cells). B. Average numbers of BrdU-positive cells per hippocampal slice. C. Average numbers of NeuN-, VGluT1-, and GAD67-positive cells merged with BrdU-positive cells per hippocampal slice. D. Confocal laser scanning microscopic images of ChAT-positive cells (arrows) in the hippocampal region of brain slices (green: ChAT-positive cells, red: PI). Abbreviations: so, stratum oriens; py, pyramidal layer of CA1; sr stratum radiatum; slm, stratum lacunosum moleculare. E. Average numbers of ChAT-positive cells in the hippocampal region per brain slice. Immunohistochemical assay and counting were carried out as described in the Materials and Methods. Five different animals were used for each treatment group. Data represent means ± SEM (^#<i>p</i> < 0.05, ^##<i>p</i> < 0.01, ^###<i>p</i> < 0.001, compared with the sham group, ^*<i>p</i> < 0.05, ^**<i>p</i> < 0.01, compared with the IBO group by the Newman-Keuls Multiple Comparison Test).