Nuclear translocation of NF-κB sub-unit.

<p><b>A</b>. Western blot analysis for the NF-κB (p65) expression level in different genetically modified HPNE cells. <b>B</b>. Western blot analysis showing constitutive nuclear expression of p65 in cells expressing oncogenic K-Ras and loss of Smad4. <b>C</b>. Effect of EGF on nuclear translocation of NF-κB (p65). HPNE/K-Ras/ShSmad4 cells are treated with EGF (50 ng/ml) and harvested at indicated time points. Western blot analyses were performed with both nuclear and cytoplasmic extracts. A parallel Coomassie blue stained gel was presented as a loading control. <b>D</b>. Nuclear translocation of p65 is partially blocked by treatment of inhibitors described above in Figure 2. <b>E</b>. NF-B mediated regulation of uPA and MMP9. ChIP assay was performed to show the binding of NF-κB to the promoters of uPA and MMP9. The details for ChIP assay and PCR primers are described in material and methods section.