Imatinib promotes p65 nuclear translocation and inhibits NF-κB target expression in a STAT3-dependent manner.

<p>(<b>A</b>) 435s/M14 cells, transfected with STAT3 siRNA, were drug treated (8 h), and nuclear fractions analyzed by Western blot. Graph is Mean±SEM from 3 independent experiments. (<b>B</b>) 435s/M14 cells stably expressing pcDNA or STAT3C were drug treated (8 h), and nuclear fractions analyzed by Western blot. Graph is Mean±SEM of 3 independent experiments. (<b>C</b>) Lysates from drug-treated pcDNA and STAT3C-expressing cells (40 h), were analyzed by Western blot. Graphs are Mean±SEM from 3 independent experiments. For all subfigures, some error bars are too small to visualize. *<i>p</i><0.05, **<i>p</i>≤0.01 (see methods).