Assessment of cellular proliferation after PCNA (Proliferating Cell Nuclear Antigen) and Tra98 immunodetection on serial sections of seminiferous tubules in organotypic culture of fresh and frozen-thawed pre-pubertal mouse testicular tissue.

<div><p>(A) Cellular proliferation assessment for organotypic culture of fresh immature mice testicular tissue assessed after 0 (1 and 4) or 11 days (2,3,5,6) of culture with RE6 (2 and 5) or RERA5 (3 and 6)</p> <p>(B) Cellular proliferation assessment for organotypic culture of frozen-thawed testicular tissue with stabilisation phase at –8°C (2 and 5) and -9°C (3 and 6) compared to fresh control organotypic culture (1 and 4)</p> <p><b>1 </b><b>to 3</b> : PCNA immunodetection</p> <p><b>4 </b><b>to 6</b> : Tra98 iommunostaining </p> <p><b>**</b> : Germ cells PCNA positive ; <b>¤¤</b> : Sertoli cell PCNA positive ; <b>^^</b> : Sertoli cell PCNA negative</p> <p>Footnotes:</p> <p><b>RE6</b> : 10^-6M retinol ; <b>RERA5</b> : 10^-5M retinoic acid and 3.3.10^-7M retinol</p>