Seminiferous tubule area assessment in organotypic culture of fresh pre-pubertal mouse testicular tissue.

<div><p>Seminiferous tubule area evolution after 0, 7, 9 and 11 days of organotypic culture on at least 30 cross-sectioned tubules after Tra98 immunostaining under the different culture conditions.</p> <p>The results are represented as the mean ± SEM with n=6.</p> <p>Footnotes:</p> <p>T: basal culture medium without retinoid; <b>RARE</b>: 3.3.10^-7M RA and 3.3.10^-7M RE; <b>RARE6</b>: 3.3.10^-7M RA and 10^-6M RE; <b>RARE5</b>: 3.3.10^-7M RA and 10^-5M RE; <b>RERA6</b>: 3.3.10^-7M RE and 10^-6M RA; <b>RERA5</b>: 3.3.10^-7M RE and 10^-5M RA; <b>RA6</b>: 10^-6M RA; <b>RE6</b>: 10^-6M RE; <b>RE5</b>: 10^-5M RE; <b>RE4</b>: 10^-4M RE; <b>RE3</b>: 10^-3M RE; <b>In </b><b>vivo</b>: In vivo control.</p>