R7BP is expressed in the dLGN but is dispensable for segregation of retinogeniculate projections.

<p><b>A.</b> R7BP is expressed in WT dorsal lateral geniculate nuclei (dLGN) (traced by dashed white line) but absent from R7BP^−/− dLGN. <b>B-C.</b> Representative fluorescent labeling of contralateral (red) and ipsilateral (green) retinogeniculate projections of P21 WT (B) or R7BP^−/− (C) dLGN using intraocularly injected, Alexa-conjugated cholera toxin B subunit (CTB) as anterograde tracers. Binarized images were thresholded at 10% to determine the area occupied by either contralateral (red), ipsilateral (green), or both (yellow) projections. <b>D.</b> Segregation of eye-specific domains is similar in WT (white bar) or R7BP^−/− (black bar) dLGN as measured by mean variance of R-value distributions from CTB-labeled retinogeniculate projections. <b>E.</b> Ablation of R7BP does not alter the area of dLGN innervated by contralateral, ipsilateral, or both projections. Error bars represent ±SEM.