Ectopic bone formation at 8 weeks.

<p>Newly formed bone was assessed by SEM in samples embedded in GMMA (A, D, G and J). BCP granules appeared in white, non-calcified tissues in black, and bone in grey (arrow head). Bar: 500 µm. Histological study of newly formed bone and tissues surrounding the material after Goldner trichrome staining as described in Materials and Methods (B, E, H and K). Bone appeared in green (arrow head) BCP: biphasic calcium phosphate. Bar; 500 µm. Magnification of region of interest (red rectangle) showing the surrounding tissues (C,F, I and L). Bone marrow niche were observed only in BG and TBM conditions. In MSC Ext OM and MSC cult OM conditions, fibrovascular tissue was only observed between the BCP granules and bone. BM: bone marrow, FVT: fibrovascular tissue. Bar: 150 µm.