Multiple alignment of caspase-3 amino acid sequences.

<p>Identical amino acids are indicated by asterisks, whereas those with high or low similarities are indicated by semicolons and dots. The putative cleavage sites at aspartic acid residues (D), which are shaded in gray, separate the pro-domain (<b><—></b>), the large subunit (<b><·····></b>) and the small subunit (<b><-----></b>) respectively. The caspase family signature and the pentapeptide active-site motif (QACRG) were boxed in a continuous and discontinuous line, respectively. The protein binding domain (GSWFI) and integrin recognition motif (RGD) are indicated by solid circles. The GenBank accession numbers of caspase-3 amino acid sequences used here are as follows: zebrafish caspase-3A NP_571952.1, zebrafish caspase-3B NP_001041531.1, Medaka caspase-3A NP_001098140.1, Medaka caspase-3B NP_001098168.1, human NP_116786.1, mouse NP_056548.2, chicken (<i>Gallus gallus</i>) NP_990056.1 and frog (<i>Xenopus laevis</i>) NP_001081225.1.