Beclin1-dot formation in LPS-induced autophagy.

<p>(A) Control or Rab39a-KD macrophages were treated with LPS for 12 h and immunostained with anti-Beclin1 antibody. An arrowhead indicates a Beclin1-dot. (B) The proportion of control or Rab39a-KD macrophages with Beclin-dot in LPS-induced autophagy is shown. Data represent the mean and SD of three independent experiments. (C) Localization of Beclin1-dot. Rab39a-KD macrophages were treated wtih LPS for 12 h and immunostained with anti-Beclin1 (red) and anti-ubiquitin (green) antibodies.