Western blot analysis of the phosphorylated and unphosphorylated forms of AKT, ERK, JNK, c-Fos, and c-Jun.

<p>SK-HEP1 liver cancer cells were treated with (at 0.5 mg/ml) or without AcFBE for 2 or 4 hrs. Immunoblotting experiments were repeated twice using GAPDH or Tubulin as controls. The duplicates were averaged and shown below each protein band, and the phosphorylated-to-unphosphorylated ratios were then calculated and shown below the treated samples. Protein bands were from the first set of immunoblotting experiment. The normalized protein level of untreated samples was set as 1.00 for comparing to their corresponding AcFBE-treated samples. 2U, untreated for 2 hrs; 2T, treated for 2 hrs; 4U, untreated for 4 hrs; 4T, treated for 4 hrs.