UA inhibited Akt-IKKα/β-NF-κB pathway in RAW 264.7 cells.

<p>Cells were pre-treated for 90 min with different concentrations of UA (70,100, 120 μM) and then treated with LPS (1 μg/ml) for 20 min. Nuclear extracts were prepared as described in materials and methods section and determined for western blotting of NF-κB and Phospho-IKKα/β proteins using specific antibodies. GAPDH served as both an internal loading control and as a nuclear fractionation control. Density ratios versus GAPDH were determined by densitometry. Results are representative of three independent experiments.