Subcellular fractionation of brains from WT mice at P0.

<p>At this stage, 4R isoforms are not expressed. 0N3R is the predominant species, and 2N3R is not detected. (<b>A</b>) Western blot analysis using the same subcellular markers as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084849#pone-0084849-g004" target="_blank">Figure 4</a>. (<b>B</b>) Western blot analysis of dephosphorylated brain fractions obtained from P0 WT mice using Tau5. (<b>C</b>) Relative ratio of the three tau isoforms in the fractions. (<b>D</b>) Significance analysis using two-way ANOVA.