Western blot analysis of ACIII expression in hippocampus, amygdala, AcbSh and SCN of WT and Bbs4^-/- mice brains normalized for actin.

<p>Note the lower level of ACIII in hippocampus and amygdala of Bbs4^-/- mice compared to WT (A) and no changes in ACIII expression in AcbSh and SCN (B). Shown is a representative western blot from three separate experiments. The data for western blot was collected from three WT and three Bbs4^-/- mice. Densitometric analysis (C) ± the SEM of the immunoblots shows the amounts of immunoreactive ACIII in the dissected areas of WT mice brains (dark gray) <i>versus</i> the corresponding dissected areas of Bbs4^-/- mice brains (light gray) using densitometric values after correction for the levels of b-actin (*p<0.05). The data for Western blot was collected from three WT and three Bbs4^-/- mice.