STRA6 mRNA expression in organs and activated T cells.

<p>STRA6 mRNA in organs (A) and activated total spleen T cells (B) was measured by RT-qPCR. WT spleen total T cells were cultured in wells coated with solid-phase anti-CD3 mAb and anti-CD28 mAb (0.5 µg/ml and 4 µg/ml, respectively, for coating) for the durations indicated. The cells were then harvested and their STRA6 mRNA levels measured by RT-qPCR. Samples were in triplicate for RT-qPCR, and means ± SD of ratios versus β-actin signals are reported. Experiments were conducted twice, and representative data are illustrated.