Transfected DNA co-localizes with STING and TBK1.

<p>(<b>A</b>) IL2/PHA PBMCs were left untreated or treated with Lipofectamine2000 for 2 hours prior to fixation and staining with anti-STING antibody (Green). (<b>B</b>) IL2/PHA PBMCs transfected with 2 µg/mL of FITC-labeled DNA as indicated for 2 hours were fixed and stained with anti-IFI16 and anti-STING antibodies (upper panel) or anti-STING and anti-TBK1 antibodies (lower panel) and visualized by confocal microscopy. STING is shown in green, IFI16 and TBK1 in red, and DNA in white. (<b>C</b>) Percentage co-localization of cytoplasmic spots positive for the respective staining was quantified by counting more than 100 cells per donor in 3 different donors. Data is shown as means +/− SD Scale bar, 5 µm. (<b>D</b>) Whole cell lysates of IL2/PHA PBMCs from 2 donors and PMA-differentiated THP1 cells treated with ssDNA (2 µg/mL) for 2 h were analyzed for levels of phosphorylated TBK1 by Western Blotting.