p-TBK1 levels in IPS-1 or STING knockdown cells.

<div><p>(A-C) siRNA for negative control, IPS-1, or STING were transfected into HeLa cells. At 48 h after transfection, cells were stimulated with HBV F1 fragment or salmon sperm DNA for 6 h. Cells were fixed and stained with Mitotracker Red and anti-p-TBK1 antibody (A), or cell lysates were prepared and subjected to SDS-PAGE and western blotting (B and C).</p> <p>(D) siRNA for IPS-1 or STING were transfected into HeLa cells At 48 h after transfection, cells were stimulated with mock, HBV F1 fragment, or salmon sperm DNA (dsDNA) for 6 h. The IFN-β mRNA expression was determined by RT-qPCR. </p>