Cytosolic DNA does not affect HIV-1 BaL replication in IL2/PHA PBMCs.

<p>(<b>A</b>) IL2/PHA PBMCs were infected with HIV-1 BaL at an MOI of 0.002, and p24 levels were measured in the supernatants after 24, 48, and 72 hours of infection. (<b>B</b>) IL2/PHA PBMCs were pretreated with AZT at increasing doses 30 min before infection with HIV-1 BaL at an MOI of 0.002. Levels of p24 were measured in the supernatants 72 hours post infection by ELISA. (<b>C, D</b>) IL2/PHA PBMCs were transfected with ssDNA (2 µg/mL) (<b>C</b>) 4 hours before or (<b>D</b>) 24 hours after infection with HIV-1. Supernatants were harvested 72 hours post infection and p24 levels measured by ELISA. Data are shown as mean of triplicates +/− SD. Similar results were obtained in three or more independent experiments. Mock, Lipofectamine.