The effect of 2c on the replication of HIV-1.

<p>(A) Jurkat cells were infected with either the NL43 wild-type (WT) or Nef-defective (ΔNef) viruses at the indicated concentrations of p24, and cultured in the presence (50 µM) or absence of 2c. AZT was also used at 5 µM. The concentration of p24 in the supernatants (at day 5, 7 or 9) was determined by ELISA. Data are shown as the mean±SD of triplicate assays and are representative of two independent experiments with similar results. (B) Peripheral blood monocyte-derived macrophages were obtained from two different donors, infected with either the JRFL wild-type (WT) or Nef-defective (ΔNef) viruses at the indicated concentrations of p24, and cultured in the presence (50 µM) or absence of 2c. AZT was also used at 5 µM. The concentration of p24 in the supernatants (at day 6, 9 or 12) was determined by ELISA. Data are shown as the mean±SD of triplicate assays. *<i>p</i><0.05.