Characterization of <i>krtt1c19e :egfp</i> transgenic larvae.

<p><b>A–D’:</b> Confocal images of eGFP expression in germline <i>krtt1c19e:egfp</i> transgenic larvae at 48 hpf (A, C–C’) and 7 dpf (B, D–D’). <b>A–B:</b> Lateral overviews of transgenic larvae indicating the <i>krtt1c19e</i> promoter drives eGFP expression in the epidermis of zebrafish larvae at all locations except the fins (extent of medial fins outlined by white line). <b>C–D’:</b> Immunofluorescent labelling of transgenic larvae cryosections demonstrates co-expression of eGFP (green; C–D’) and ΔNp63 (red; C–D’) in basal keratinocytes. Counterstaining with DAPI (blue; C–D’) and Pan-cadherin (white; C’, D’) highlights the eGFP negative region overlying EVL (demarcated by dashed lines; C’, D’). <b>E–F’’’:</b> Confocal images of the epidermis of germline <i>krtt1c19e:lyn-tdtomato; krt4:lyn-egfp</i> double transgenic larvae at 72 hpf (E–E’’) and 7 dpf (F–F’’’) immunofluorescently stained for eGFP (green; E’–E’’, F’, F’’’), tdTomato (red; E, E’’, F, F’’’), ΔNp63 (white; E, E’’) and ZO-1 (white; F’’–F’’’). The expression of membrane bound tdTomato delineates the ΔNp63 positive basal keratinocytes from the eGFP expressing ZO-1 positive EVL cells.