Generation of <i>Tg(hsp70:sdf1a-nanos3, EGFP)</i> zebrafish.

<p>(A) Diagram of the plasmid construct used to generate the transgenic line. A 1.5-kb fragment of the <i>hsp70</i> promoter controls expression of zebrafish <i>sdf1a</i> and <i>EGFP</i>. Three different EGFP expression patterns were observed in F1 offspring obtained from different founder fish. (B,C) strong ubiquitous expression, (D,E) weak expression throughout the body, (F,G) strong expression in the brain and weak in the body. The strongly expressing larvae were raised to sexual maturity and used to establish the homozygous line. Scale bar = 200 µm.