<p>Crypts were grown in culture media containing Rspo1, with or without ISEMF CM, and assessed after 7 days of culture. (A) Average area of an enteroid grown with ISEMF CM was compared to without conditioned media (n = 4). (B,C) Representative micrographs of an enteroid grown without CM (B) and with ISEMF CM (B). The scale bar represents 100 µm. Asterisk indicates p<0.05 when compared to <i>No CM</i>.</p
At the end of the incubation period, colonoids were examined by light microscopy after staining with...
<p>Human small intestinal crypts cultured on murine myofibroblasts in the presence of Wnt3a and FGF1...
The epithelium of the gastrointestinal tract is constantly renewed as it turns over. This process is...
<p>Crypts with and without ISEMFs were grown in culture media that contained Rspo1 and assessed afte...
<p>Crypts with and without ISEMFs were grown in culture media that contained Rspo1 and assessed afte...
<p>Enteroid forming efficiency was measured in mono- and co-cultures with ISEMFs, with and without e...
<p>(A) The total cross-sectional area of the enteroids per well was measured to quantify the growth ...
<p>Human crypts cultured on murine myofibroblasts in the presence of Wnt3a and FGF10 were processed ...
<p>The <i>in vitro</i> isolation, growth and maintenance of enteroids derived from porcine intestina...
<p>(<b>A</b>) GFP epithelium after 4-day co-culture with non-GFP IMC. (<b>B</b>) Proliferation (Ki67...
<p>To determine the biochemical characteristics of the factors that simulate colon crypt formation <...
<p>Micrographs of human small intestinal crypts that were cultured on human infant myofibroblasts fo...
<p>Conditioned Medium (CM) from WEHI-YH2 cells (YH2CM) was collected from confluent cultures incubat...
<p>Different proportions (2.5% to 50%, v/v) of ten-fold concentrated YH2CM conditioned media (cYH2CM...
<p>(A) Rspo1 and Rspo2 mRNA expression was measured by qPCR in ISEMFs that were supportive or non-su...
At the end of the incubation period, colonoids were examined by light microscopy after staining with...
<p>Human small intestinal crypts cultured on murine myofibroblasts in the presence of Wnt3a and FGF1...
The epithelium of the gastrointestinal tract is constantly renewed as it turns over. This process is...
<p>Crypts with and without ISEMFs were grown in culture media that contained Rspo1 and assessed afte...
<p>Crypts with and without ISEMFs were grown in culture media that contained Rspo1 and assessed afte...
<p>Enteroid forming efficiency was measured in mono- and co-cultures with ISEMFs, with and without e...
<p>(A) The total cross-sectional area of the enteroids per well was measured to quantify the growth ...
<p>Human crypts cultured on murine myofibroblasts in the presence of Wnt3a and FGF10 were processed ...
<p>The <i>in vitro</i> isolation, growth and maintenance of enteroids derived from porcine intestina...
<p>(<b>A</b>) GFP epithelium after 4-day co-culture with non-GFP IMC. (<b>B</b>) Proliferation (Ki67...
<p>To determine the biochemical characteristics of the factors that simulate colon crypt formation <...
<p>Micrographs of human small intestinal crypts that were cultured on human infant myofibroblasts fo...
<p>Conditioned Medium (CM) from WEHI-YH2 cells (YH2CM) was collected from confluent cultures incubat...
<p>Different proportions (2.5% to 50%, v/v) of ten-fold concentrated YH2CM conditioned media (cYH2CM...
<p>(A) Rspo1 and Rspo2 mRNA expression was measured by qPCR in ISEMFs that were supportive or non-su...
At the end of the incubation period, colonoids were examined by light microscopy after staining with...
<p>Human small intestinal crypts cultured on murine myofibroblasts in the presence of Wnt3a and FGF1...
The epithelium of the gastrointestinal tract is constantly renewed as it turns over. This process is...