FG-4497 increases HIF-α and PHD3 protein abundance in endothelial cells and astrocytes.

<p>Mouse cerebrovascular bEnd.3 cells (<b>A</b>) and primary murine astrocytes (<b>B</b>) were treated with either 1 mM DMOG or FG-4497 (5, 20 and 50 µM) for 6 hours. As DMOG and FG-4497 were dissolved in DMSO, 0.2% DMSO was used as a control. Then, cellular proteins were isolated and HIF-1α, HIF-2α and PHD3 abundance was quantified by Western blot. Values are normalized to β-actin and expressed as fold change to CTRL (control = non-treated cells). Significant differences determined by one-way ANOVA combined with Bonferroni post-test are indicated with *(<i>p</i><0.05), **(<i>p</i><0.01) or ***(<i>p</i><0.001). <i>N</i> = 3.