Effect of FG-4497 on the VEGF expression in endothelial cells exposed to ischemic conditions.

<p>Mouse cerebrovascular bEnd.3 cells were treated with 20 µM FG-4497 for 6 hours under OGD or basal conditions. Subsequently, RNA was isolated and gene expression was analyzed by real-time PCR. Values are normalized to <i>Rps12</i> and expressed as fold change to untreated cells cultured under non-ischemic conditions. Significant differences determined by one-way ANOVA combined with Bonferroni post-test are indicated with **(<i>p</i><0.01). <i>N</i> = 3.