FG-4497 sustains subcellular localization of occludin and ZO-1 in cerebral blood capillaries upon ischemic stroke.

<p>100/kg FG-4497 or an equal volume of 0.9% NaCl was applied intraperitoneally to adult C57BL/6 mice. After 6 hours mice underwent 60 min of MCAO followed by 24 hours reperfusion. Subsequently, brains were removed, coronal cryosections were prepared and immunofluorescent detection of occludin and ZO-1 was performed. Disruptions (gaps; denoted by white arrowheads) of the regular occludin and ZO-1 localization pattern, respectively were counted in at least three randomly chosen blood vessels localized within the peri-infarct region per animal, and the number of gaps per vessel was calculated. Significant differences determined by unpaired two-tailed t-test are indicated with **(<i>p</i><0.01). <i>N</i> = 4 (per group).